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Book Chapters

  1. Wu Y, Gallego-Perez D, Lee LJ. Microwell Array-Mediated Delivery of Lipoplexes Containing Nucleic Acids for Enhanced Therapeutic Efficacy. In: RNA Interference - Challenges and Therapeutic Opportunities. Edited by Mouldy S. Springer. 2014.
  2. Gallego-Perez D, Pelaez-Vargas A, Gomez D, Fernandes MH, Hansford D, Monteiro FJ. Proliferation of Human Bone Marrow Stem Cells for Craniofacial applications. In: Stem Cells and Cancer Stem Cells: Therapeutic Applications in Disease and Injury. Edited by Hayat MA. Springer. 2012.
  3. Pelaez-Vargas A, Gallego-Perez D, Higuita-Castro N, Carvalho A, Grenho L, Arismendi JA, Fernandes MH, Ferraz MP, Hansford DJ, Monteiro FJ. Micropatterned Coatings for Guided Tissue Regeneration in Dental Implantology. In: Cell Interaction. Edited by Gowder S. InTech. 2012.


  1. Gallego-Perez D, Chang L, Shi J, Ma J, Kim S-H, Zhao X, Malkoc V, Wang X, Minata M, Kwak KJ, Wu Y, Lafyatis G, Lu W, Hansford DJ, Nakano I, Lee LJ. On-chip clonal analysis of glioma stem cell motility and therapy resistance. Nano Letters 2016. DOI: 10.1021/acs.nanolett.6b00902.
  2. Gu S-Q, Gallego-Perez D, McClory S, Shi J, Han J, Lee LJ, Schoenberg D. The human PMR1 endonuclease stimulates cell motility by down regulating miR-200 family microRNAs. Nucleic Acids Research 2016. DOI: 10.1093/nar/gkw497.
  3. Kim S-H, Ezhilarasan R, Phillips E, Gallego-Perez D, Sparks A, Taylor D, Ladner K, Furuta T, Sabit H, Chhipa R, Cho J-H, Mohyeldin A, Beck S, Kurozumi K, Kuroiwa T, Iwata R, Asai A, Kim J, Sulman EP, Cheng S-Y, Lee LJ, Nakada M, Guttridge D, DasGupta B, Goidts V, Bhat KP, Nakano I. Serine/Threonine Kinase MLK4 Determines Mesenchymal Identity in Glioma Stem Cells in an NF-κB-dependent Manner. Cancer Cell 2016; 29:201-213.
  4. Gallego-Perez D, Otero JJ, Czeisler C, Ma J, Ortiz C, Gygli P, Catacutan F, Gokozan H, Cowgill A, Sherwood T, Ghatak S, Malkoc V, Zhao Z, Liao W-C, Gnyawali S, Wang X, Adler AF, Leong K, Wulff B, Wilgus TA, Askwith C, Khanna S, Rink C, Sen CK, Lee LJ. Deterministic Transfection Drives Efficient Nonviral Reprogramming and Uncovers Reprogramming Barriers. Nanomedicine 2015. DOI:
  5. Malkoc V, Gallego-Perez D, Nelson T, Lannutti JJ, Hansford DJ. Controlled neuronal cell patterning and guided neurite growth on micropatterned nanofiber platforms. Journal of Micromechanics and Microengineering 2015. 25: 125001.
  6. Bertani P, Lu W, Chang L, Gallego-Perez D, Lee LJ, Chiang C, Muthusamy N. Bosch etching for the creation of a 3D nanoelectroporation system for high throughput gene delivery. JVSTB 2015/ 33: 06F903.
  7. Zhao X, Huang X, Wang X, Wu Y, Eisfeld A-K, Schwind S, Gallego-Perez D, Boukany PE, Marcucci GI, Lee LJ. Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia. Advanced Science 2015. DOI: 10.1002/advs.201500111.
  8. Chang L, Bertani P, Gallego-Perez D, Yang Z, Chen F, Chang C, Malkoc V, Kuang T, Gao K, Lee LJ, Lu W. 3D Nanochannel Electroporation for High-throughput Cell Transfection with High Uniformity and Dosage Control. Nanoscale 2015; 8:243-252.
  9. Gallego-Perez D, Chang L, Zhao X, Bertani P, Yang Z, Chiang C-L, Malkoc V, Shi J, Sen CK, Odonnell L, Yu J, Lu W, Lee LJ. Dielectrophoresis-assisted 3D nanoelectroporation for non-viral cell transfection in adoptive immunotherapy. Lab Chip 2015. 15: 3147-3153.
  10. Holfinger SJ, Reinhardt JW, Reen R, Schultz KM, Passino KM, Ackerman WE, Kniss DA, Sander LM, Gallego-Perez D, Gooch KJ. Pancreatic Epithelial Cells Form Islet-Like Clusters in the Absence of Directed Migration. Cellular and Molecular Bioengineering 2015; 8:496-506.
  11. Zhao X, Wu Y, Gallego-Perez D, Kwak K, Gupta C, Ouyang X, Lee LJ. Effect of Non-Endocytic Uptake of Nanoparticles on Human Bronchial Epithelial Cells. Anal Chem 2015. 17: 3208-3215.
  12. Wang X, Huang X, Yang Z, Gallego-Perez D, Ma J, Zhao X, Xie J, Nakano I, Lee LJ. Targeted Delivery of Tumor Suppressor MicroRNA-1 by Transferrin-Conjugated Lipopolyplex Nanoparticles to Patient-Derived Glioblastoma Stem Cells. Curr Pharm Biotechno 2014; 15:839-846
  13. Chang L, Howdyshell M, Liao W-C, Chiang C-L, Gallego-Perez D, Yang Z, Lu W, Byrd JC, Muthusamy N, Lee LJ, Sooryakumar R. Magnetic Tweezers-based 3D Microchannel Electroporation for High-Throughput Gene Transfection in Living Cells. Small 2014. 11: 1818-1828.
  14. Xie P, He P, Yen Y-C, Kwak K, Gallego-Perez D, Chang L, Liao W-C, Yi A, Lee LJ. Rapid hot embossing of polymer microstructures using carbide-bonded graphene coating on silicon stampers. Surface and Coatings Technology 2014. 15:174-180.
  15. Benavente-Babace A, Gallego-Perez D, Hansford DJ, Arana S, Perez-Lorenzo E, Mujika M. Single-cell trapping and selective treatment via co-flow within a microfluidic platform. Biosensors and Bioelectronics 2014; 61:298–305.
  16. Gupta C, Liao W-C, Gallego-Perez D, Castro CE, Lee LJ. DNA translocation through short nanofluidic channels under asymmetric pulsed electric field. Biomicrofluidics 2014; 8:024114.
  17. Gao K, Li L, He L, Hinkle K, Wu Y, Ma J, Chang L, Zhao X, Gallego-Perez D, Eckardt S, Mclaughlin J, Liu B, Farson DF, Lee LJ. Design of a Microchannel-Nanochannel-Microchannel Array Based Nanoelectroporation System for Precise Gene Transfection. Small 2014; 10:1015–1023.
  18. Huang W, Yu J, Kwak KJ, Gallego-Perez D, Liao W, Yang H, Ouyang X, Li L, Lu W, Lafyatis GP, Lee LJ. Atomic Carbide Bonding Leading to Superior Graphene Networks. Advanced Materials 2013; 25: 4668-4672.
  19. Gallego-Perez D, Pelaez-Vargas A, Carvalho A, Fernandes MH, Hansford DJ, Monteiro FJ. Effects of density of anisotropic micro-stamped silica thin films on guided bone tissue regeneration – in vitro study. J Biomed Mater Res B Appl Biomater 2013; 101:762-769.
  20. Wu Y, Cavanaugh-Terp M, Kwak KJ, Gallego-Perez D, Nana-Sinkam SP, Lee LJ. Surface-Mediated Nucleic Acid Delivery by Lipoplexes Prepared in Microwell Arrays. Small 2013; 9:2358-2367.
  21. Fei Z, Wu Y, Sharma S, Gallego-Perez D, Higuita-Castro N, Hansford DJ, Lannutti J, Lee LJ. Gene Delivery to Cultured Embryonic Stem Cells Using Nanofiber-Based Sandwich Electroporation. Anal Chem 2013; 85:1401–1407.
  22. Dinan B, Gallego-Perez D, Lee H, Hansford DJ, Akbar SA. Thermally Grown TiO2 Nanowires for Improving Cell Growth and Proliferation on Titanium Based Materials. Ceram Int 2012; 39: 5949–5954.
  23. Higuita-Castro N, Gallego-Perez D, Love K, Sands MR, Kaletunc G, Hansford DJ. Soft Lithography-Based Fabrication of Biopolymer Microparticles for Nutrient Microencapsulation. Ind. Biotechnol 2012; 8: 365-371.
  24. Gallego-Perez D, Higuita-Castro N, Denning L, DeJesus J, Dahl K, Sarkar A, Hansford DJ. Microfabricated mimics of in vivo structural cues for the study of guided tumor cell migration. Lab Chip 2012; 12: 4424-4432.
  25. Carvalho A, Pelaez-Vargas A, Gallego-Perez D, Grenho L, Fernandes MH, De Aza AH, Ferraz MP, Hansford DJ, Monteiro FJ. Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration. Dent Mater 2012; 28:1250-1260.
  26. Wang F, He H, Wang X, Li Z, Gallego-Perez D, Guan J, James Lee LJ. Micropatterned Thermoresponsive Surfaces by Polymerization of Monomer Crystals: Modulating Cellular Morphology and Cell–Substrate Interactions. Anal Chem 2012; 84:9439–9445.
  27. Gallego-Perez D, Higuita-Castro N, Reen RK, Palacio-Ochoa M, Sharma S, Lee LJ, Lannutti JJ, Hansford DJ, Gooch KJ. Micro/nanoscale technologies for the development of hormone-expressing islet-like cell clusters. Biomed Microdevices 2012; 14:779-789.
  28. Carvalho A, Pelaez-Vargas A, Gallego-Perez D, Fernandes MH, Hansford DJ, Monteiro FJ. Micropatterned bioactive thin films for guided bone regeneration. Eur Cell Mater 2012; 23 (Suppl 2): 13.
  29. Pelaez-Vargas A, Gallego-Perez D, Fernandes MH, Hansford D, Monteiro FJ. Microstructured coatings to study the behavior of osteoblast-like cells on hard materials. Bone 2011; 48 (Suppl 2): S105-S106.
  30. Carvalho A, Pelaez-Vargas A, Gallego-Perez D, Fernandes MH, Hansford D, Monteiro FJ. Adhesion and proliferation of mesenchymal stem cells on micropatterned thin films modified with nanohydroxyapatite particles. Bone 2011; 48 (Suppl 2): S106.
  31. Higuita-Castro N, Gallego-Perez D, Pelaez-Vargas A, García Quiroz F, Posada OM, Lopez LE, Sarassa CA, Agudelo-Florez P, Monteiro FJ, Litsky AS, Hansford DJ. Reinforced Portland cement porous scaffolds for load- bearing bone tissue engineering applications. J Biomed Mater Res B Appl Biomater 2011; 100B:501-507.
  32. Gallego-Perez D, Higuita-Castro N, Garcia Quiroz F, Posada OM, Lopez LE, Litsky AS, Hansford DJ. Portland cement for bone tissue engineering: effects of processing and metakaolin blends. J Biomed Mater Res B Appl Biomater 2011; 98B:308-315.
  33. Pelaez-Vargas A, Gallego-Perez D, Magallanes-Perdomo M, Fernandes MH, Hansford DJ, De Aza AH, Pena P, Monteiro FJ. Isotropic micropatterned silica coatings on zirconia induce guided cell growth for dental implants. Dent Mater 2011; 27:581-589.
  34. Pelaez-Vargas A, Gallego-Perez D, Ferrell N, Fernandes MH, Hansford D, Monteiro FJ. Early spreading and propagation of bone marrow stem cells on isotropic and anisotropic topographies of silica thin films produced via microstamping. Microsc Microanal 2010; 16:670-676.
  35. Gallego-Perez D, Ferrell NJ, Higuita-Castro N, Hansford DJ. Versatile methods for the fabrication of polyvinylidene fluoride microstructures. Biomed Microdevices 2010; 12:1009-1017.
  36. Sabbani S, Gallego-Perez D, Nagy A, Waldman WJ, Hansford D, Dutta PK. Synthesis of silver-zeolite films on micropatterned porous alumina and its application as an antimicrobial substrate. Microporous Mesoporous Mater 2010; 135:131-136.
  37. Garcia Quiroz F, Posada OM, Gallego-Perez D, Higuita-Castro N, Sarassa C, Hansford DJ, Agudelo-Florez P, Lopez LE. Housekeeping gene stability influences the quantification of osteogenic markers during stem cell differentiation to the osteogenic lineage. Cytotechnology 2010; 62:109-120.
  38. Ferrell N, Gallego-Perez D, Higuita-Castro N, Butler RT, Reen RK, Gooch KJ, Hansford DJ.  Vacuum-assisted cell seeding in a microwell cell culture system. Anal Chem 2010; 82:2380-2386.
  39. Gallego-Perez D, Higuita-Castro N, Sharma S, Reen RK, Palmer AF, Gooch KJ, Lee LJ, Lannutti JJ, Hansford DJ. High throughput assembly of spatially controlled 3D cell clusters on a micro/nanoplatform. Lab Chip 2010; 10:775-782.
  40. Pelaez-Vargas A, Gallego-Perez D, Higuita-Castro N, Hansford DJ, Monteiro FJ. Zirconia Microtextured Surfaces Using Soft Lithography. Journal of Dental Research 2010; 89 (Spec Iss B): 4004.
  41. Pelaez-Vargas A, Gallego-Perez D, Higuita-Castro N, Hansford DJ, Monteiro FJ. Microstamped Silica Films on Curved Zirconia Surfaces. Journal of Dental Research 2010; 89 (Spec Iss B):4005.
  42. Pelaez-Vargas A, Gallego-Perez D, Fernandes MH, Hansford DJ, Monteiro FJ. Anisotropic Microtextured Silica Thin Films on Zirconia. Journal of Dental Research 2010; 89 (Spec Iss B):1391.
  43. Pelaez-Vargas A, Higuita-Castro N, Gallego-Perez D, Hansford DJ, Monteiro FJ. Portland Particulated Silica Coatings – Comparison between Two Coating Techniques. Journal of Dental Research 2010; 89 (Spec Iss B):4006.
  44. Pelaez-Vargas A, Gallego-Perez D, Ferrell N, Fernandes MH, Hansford DJ, Magallanes-Perdomo M, De Aza A, Pena P, Monteiro FJ. Microfabricated SiO2 Thin Films on Structural Dental Ceramics. Bioceramics 2009; 22:779.
  45. Xu J, Dapino MJ, Gallego-Perez D, Hansford D. Microphone based on Polyvinylidene Fluoride (PVDF) micro-pillars and patterned electrodes. Sens Actuators A 2009; 153:24-32.
  46. Gallego D, Higuita N, Garcia F, Ferrell N, Hansford DJ. Bioactive Coatings on Portland cement Substrates: Surface precipitation of Apatite-like Crystals. Mater Sci Eng C 2008; 28:353-358.
  47. Gallego D, Ferrell N, Sun Y, Hansford DJ. Multilayer Micromolding of Degradable Polymer Tissue Engineering Scaffolds. Mater Sci Eng C 2008; 28:347-352.